Factors Affecting the Rate of Enzyme Activity (Leaving Cert Biology): Flashcards

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Factors Affecting the Rate of Enzyme Activity
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Three factors affecting enzyme activity rate

Temperature, pH, enzyme inhibitors

Optimum temperature

Temperature at which an enzyme works best

Enzyme activity rate at 0°C

Zero (water freezes, molecules cannot move)

Effect of rising temperature on enzyme activity

Rate increases (molecules move faster, more collisions)

Temperature above which human enzymes denature

Above 37°C

Denaturation

Active site destroyed permanently; enzyme stops working

Optimum temperature range for plant enzymes

25°C to 35°C

Optimum pH

pH at which an enzyme works best

pH scale range

0 to 14

Optimum pH range for many enzymes

pH 6 to 8

Purpose of buffer solution

Keeps pH constant in experiments

Enzyme inhibitors

Chemicals that attach to enzyme and destroy its shape

Cyanide as enzyme inhibitor

Blocks enzymes involved in respiration

Heavy metal enzyme inhibitors (examples)

Mercury and lead

Effect of extreme pH on enzymes

Enzyme becomes denatured (active site changes permanently)

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