Action and Specificity (Leaving Cert Biology): Flashcards

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Action and Specificity
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Active site of enzyme

Region where substrate binds and reactions occur

What % of enzyme is the active site?

10-20%

Shape of active site relative to substrate

Complementary

Induced fit model

Active site changes shape slightly when substrate binds

Enzyme-substrate complex

Enzyme and substrate bonded together at active site

Substrate molecules enzyme can process per second

Several thousand

Enzyme specificity

Enzyme works with only one type of substrate

Denaturation

Permanent loss of enzyme function due to shape change

Is denaturation reversible?

No, it's permanent

Optimal temperature for human enzymes

Around 37°C

Temperature causing permanent enzyme damage

Above 40°C

Substrate that catalase breaks down

Hydrogen peroxide

Products of catalase reaction

Water and oxygen

Examples of where catalase is found

Radishes, celery, potatoes, most living organisms

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