Factors Affecting the Rate of Enzyme Activity (Leaving Cert Biology): Flashcards

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Factors Affecting the Rate of Enzyme Activity
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Three factors affecting enzyme action rate

Temperature, pH, enzyme inhibitors

Optimum temperature

Temperature at which an enzyme works best

Enzyme activity at 0°C

Zero - molecules cannot move

Effect of rising temperature on enzyme activity

Increases rate as molecules collide more often

Temperature above which human enzyme activity decreases

Above 37°C

Denaturation

Active site destroyed; enzyme stops working permanently

Optimum temperature range for plant enzymes

25°C to 35°C

Optimum pH

The pH at which an enzyme works best

pH scale range

0 to 14

Optimal pH range for many enzymes

pH 6 to 8

Effect of extreme pH on enzymes

Denaturation - active site changes shape permanently

Purpose of buffer solution in experiments

Keep pH constant

Enzyme inhibitors

Chemicals that attach to enzyme and destroy its shape

Examples of enzyme inhibitors

Cyanide, mercury, lead, sarin

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