Bioprocessing with Immobilised Cells (Leaving Cert Biology): Flashcards
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Bioprocessing with immobilised cells
Bioprocessing with immobilised cells
Using immobilised cells for enzyme-controlled reactions producing useful products
Immobilised enzyme definition
Immobilised enzyme definition
Enzyme attached to an inert material
Chemical mixed with cells for immobilisation
Chemical mixed with cells for immobilisation
Sodium alginate
Chemical that causes alginate to form beads
Chemical that causes alginate to form beads
Calcium chloride
What calcium chloride does to alginate
What calcium chloride does to alginate
Forms beads around cells
Function of pores in immobilisation beads
Function of pores in immobilisation beads
Allow substrates in and products out
Examples of bioprocessed products
Examples of bioprocessed products
Solvents, amino acids, vitamins, drugs, enzymes, biogas
Use of immobilised E. coli
Use of immobilised E. coli
Water treatment to detoxify sewage
Stability advantage of immobilised cells
Stability advantage of immobilised cells
Cells remain very stable and are not harmed
Separation advantage of immobilised cells
Separation advantage of immobilised cells
Easily separated from products
Reusability of immobilised cells
Reusability of immobilised cells
Can be reused many times, cost-effective
Why use immobilised cells over enzymes
Why use immobilised cells over enzymes
Enzymes more difficult and expensive to prepare