Tissue Culturing (Leaving Cert Biology): Flashcards

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Tissue Culturing
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What is tissue culturing?

Growing new tissues/organs/plants from small tissue pieces

Why aseptic conditions in tissue culture?

Sterile conditions to prevent bacterial/fungal contamination

What nutrient medium contains

Nutrients, growth hormones, sometimes antibiotics

What does totipotency mean?

Each plant cell can grow into a complete new plant

Plant tissue culture use: cloning

Rapidly produces large numbers of genetically identical plants

Plant tissue culture use: conservation

Conserves rare or endangered plant species

Plant tissue culture use: health

Produces disease-free plants

Medical use: antibodies

Producing monoclonal antibodies for diagnosis and treatment

Tissue culture advantage: timing

Can be done year-round

Tissue culture disadvantage: cost

Expensive

Tissue culture disadvantage: genetics

Risk of reduced genetic variation in cloned populations

Medical applications of tissue culture

IVF, skin grafts, vaccine production

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