Bioprocessing with Immobilised Cells (Leaving Cert Biology): Flashcards

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Bioprocessing with Immobilised Cells
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Bioprocessing w/ immobilised cells

Using immobilised cells for enzyme-controlled reactions

Immobilised enzyme definition

Enzyme attached to an inert material

First substance mixed with cells to immobilise

Sodium alginate

Substance that forms beads around cells

Calcium chloride

What beads do to cells

Immobilise cells while allowing substrates/products through

What do pores allow to enter

Substrates

Why cells can't pass through pores

Pores are too small for cells

Use of immobilised E. coli

Water treatment to detoxify sewage

Stability advantage of immobilised cells

Cells remain very stable

Separation advantage of immobilised cells

Easily separated from products

Reusability of immobilised cells

Can be reused many times

Advantage of immobilised cells over enzymes

Enzymes more difficult & expensive to prepare

Example products from immobilised cells

Biogas, vitamins, drugs, amino acids, enzymes

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