Tissue Culturing (Leaving Cert Biology): Flashcards

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Tissue Culturing
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Tissue culturing definition

Growing tissues/organs/plants from tissue pieces in sterile environment

Aseptic conditions in tissue culturing

Sterile conditions to prevent bacterial/fungal contamination

Components of nutrient medium

Nutrients, growth hormones, sometimes antibiotics

Totipotency in plant cells

Each cell can grow into a complete new plant

Plant tissue culturing - cloning benefit

Rapidly produces large numbers of genetically identical plants

Plant tissue culturing - conservation use

Conserves rare or endangered plant species

Plant tissue culturing - disease benefit

Produces disease-free plants

Medical use - monoclonal antibodies

Producing antibodies for diagnosis and treatment

Tissue culturing advantage - timing

Can be done year-round

Tissue culturing disadvantage - cost

Expensive

Tissue culturing disadvantage - genetics

Risk of reduced genetic variation in cloned populations

Medical applications of tissue culturing

IVF, skin grafts, vaccine production

Plant tissue culturing technique name

Micro-propagation

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