DNA Profiling (Leaving Cert Biology): Flashcards

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DNA Profiling
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DNA profiling definition

Method of making unique pattern of DNA bands to distinguish DNA

Sources for DNA extraction

Blood, hair follicle, bodily fluid

Chemical used to extract DNA

Detergent

What detergent dissolves in DNA extraction

Cell membranes (lipids)

Enzymes that cut DNA into fragments

Restriction enzymes

Cut sections of DNA are called

Restriction fragments

Method to separate restriction fragments

Gel electrophoresis

Gel electrophoresis separates fragments by

Size

Which fragments move faster in gel

Smaller fragments

How DNA profile is viewed

Under UV light

What DNA profile looks like

Bar code

Crime use of DNA profiling

Link person to crime/crime scene

Medical use of DNA profiling

Paternity test (establish if man is father of child)

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