Applications (Leaving Cert Biology): Flashcards

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Applications
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CRISPR-Cas9 function

Molecular scissors for precise DNA cuts at specific locations

Two components of CRISPR system

Guide RNA and Cas9 enzyme

Two outcomes after CRISPR cuts DNA

Gene disruption or gene insertion

3 medicines from GM bacteria

Insulin, human growth hormone, antibiotics

GM goats/sheep pharmaceutical use

Produce human proteins in their milk

Gene therapy definition

Replacing faulty genes with functioning versions

3 gene therapy targets

Cystic fibrosis, sickle cell anaemia, haemophilia

3 GM crop capabilities

Resist herbicides, produce toxins against pests, improve nutrition

2 environmental uses of GM bacteria

Break down pollutants, extract metals from soil

DNA profiling purpose

Creates unique genetic signature for identification

DNA profiling developer & year

Sir Alec Jeffries, 1984

DNA profile match probability (unrelated)

Less than 1 in a billion

Bioinformatics definition

Combines computer science, statistics & biology

GenBank purpose

Stores genetic sequences for researchers worldwide

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