DNA Profiling and Sequencing (Leaving Cert Biology): Flashcards
📚Flashcards
Practise the cards
13 cards from this deck
ShowHide
Practise the cards
13 cards from this deck
Alternative name for DNA profiling
Alternative name for DNA profiling
DNA fingerprinting
Exception to unique DNA profiles
Exception to unique DNA profiles
Identical twins
PCR acronym meaning
PCR acronym meaning
Polymerase Chain Reaction
PCR purpose in DNA profiling
PCR purpose in DNA profiling
Amplifies DNA to make millions of copies for analysis
PCR cycles & copies produced
PCR cycles & copies produced
~30 cycles → ~1 billion copies
Taq polymerase function in PCR
Taq polymerase function in PCR
Builds new DNA strands
Restriction enzymes function
Restriction enzymes function
Cut DNA at specific sequence patterns into fragments
Gel electrophoresis separates DNA by
Gel electrophoresis separates DNA by
Size (smaller fragments move faster & further)
DNA charge in gel electrophoresis
DNA charge in gel electrophoresis
Negative (moves to positive electrode)
DNA sequencing determines
DNA sequencing determines
Exact order of nucleotides (A, T, G, C)
Sanger sequencing inventor
Sanger sequencing inventor
Fred Sanger (won Nobel Prize twice)
Next-generation sequencing advantages
Next-generation sequencing advantages
Faster, more accurate, cheaper than traditional methods
Original Human Genome Project time & cost
Original Human Genome Project time & cost
13 years, ~£2.5 billion