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13 cards from this deck
Manipulating genetic material to modify organisms in ways that don't occur naturally
Genetic modification, recombinant DNA technology, or gene editing
DNA combining genetic material from different sources
Genetically Modified Organism
Small circular DNA in bacteria used as gene vehicles
DNA isolation, Cutting, Ligation, Transformation, Expression
Cut DNA at specific sequences like molecular scissors
Must use same enzyme to cut both gene and plasmid
Joins DNA pieces together like molecular glue
Recombinant plasmid entering bacterial cells
Gene transcribed & translated to produce protein
Guide RNA (gRNA) and Cas9 enzyme
Emmanuelle Charpentier and Jennifer Doudna
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