The Process of Genetic Engineering (Leaving Cert Biology): Flashcards
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Genetic engineering definition
Genetic engineering definition
Artificial alteration or manipulation of genes
Recombinant DNA
Recombinant DNA
Altered DNA after cutting and pasting genes
Transgenic organism
Transgenic organism
Organism with DNA transferred from different species
Mnemonic for genetic engineering steps
Mnemonic for genetic engineering steps
I Caught Leprosy Through Careless Experimentation (Isolation, Cutting & Ligation, Transformation, Cloning, Expression)
Isolation in genetic engineering
Isolation in genetic engineering
Process of removing DNA from a cell
Role of detergent in isolation
Role of detergent in isolation
Breaks down the lipid membrane
Role of protease in isolation
Role of protease in isolation
Breaks down proteins surrounding the DNA
Enzyme used for cutting DNA
Enzyme used for cutting DNA
Restriction enzymes
Enzyme used for ligation
Enzyme used for ligation
DNA ligase
Plasmid role in genetic engineering
Plasmid role in genetic engineering
Acts as a vector (circular bacterial DNA)
Transformation in genetic engineering
Transformation in genetic engineering
Uptake of recombinant DNA into bacterial cell
Cloning in genetic engineering
Cloning in genetic engineering
Production of identical copies of a cell
Role of antibiotic in cloning
Role of antibiotic in cloning
Identifies cells with plasmids on agar
Role of radioactive probe in cloning
Role of radioactive probe in cloning
Identifies colonies containing target gene
Expression in genetic engineering
Expression in genetic engineering
Host cell produces product of inserted gene