Bioprocessing with Immobilised Cells (Leaving Cert Biology): Quizzes

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Bioprocessing with Immobilised Cells
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What are immobilised cells used for in bioprocessing?

Enzyme-controlled reactions

What is an immobilised enzyme?

An enzyme attached to an inert material

What substance are cells mixed with during immobilisation?

Sodium alginate

What is the yeast/alginate mixture soaked in?

Calcium chloride

What does calcium chloride cause the alginate to do?

Form beads around cells

What can pass through the pores in the beads?

Substrates and products

Why can't yeast cells escape from the beads?

The pores are too small

Which of these is produced using immobilised cells?

Biogas

What is immobilised E. coli used for?

Detoxifying sewage in water treatment

How does immobilisation affect cell stability?

Cells remain very stable

Why are immobilised cells cost-effective?

They can be reused many times

Why use immobilised cells instead of immobilised enzymes?

Enzymes are more difficult to prepare

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