Genetic Engineering (LC 2027) (Leaving Cert Biology): Quizzes

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The Process of Genetic Engineering
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What is genetic engineering?

Artificial alteration of genes

What is recombinant DNA?

Altered DNA from cutting and pasting

What is a transgenic organism?

Organism with DNA from another species

What is the role of detergent in DNA isolation?

Breaks down the lipid membrane

What breaks down proteins surrounding DNA during isolation?

Protease

Which enzyme cuts DNA to remove a gene?

Restriction enzyme

What is a plasmid?

Circular bacterial DNA

What does DNA ligase do?

Joins cut ends of gene with vector DNA

What is transformation in genetic engineering?

Uptake of recombinant DNA into a cell

Why is antibiotic used in the cloning step?

Only plasmid-containing cells survive

What identifies colonies containing the target gene during cloning?

Radioactive probe

What is expression in genetic engineering?

Host cell produces gene product

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