DNA Profiling and Sequencing (Leaving Cert Biology): Quizzes

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DNA Profiling and Sequencing
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What is another name for DNA profiling?

DNA fingerprinting

Who would have identical DNA profiles?

Identical twins

What is the purpose of PCR in DNA profiling?

Makes millions of copies of DNA

Which enzyme is essential for the PCR process?

Taq polymerase

What temperature is used in the denaturation step of PCR?

94°C

How many DNA copies are produced after 30 PCR cycles?

Roughly one billion

What is the function of restriction enzymes?

Cut DNA at specific sequences

Why does DNA move during gel electrophoresis?

DNA is negatively charged

In gel electrophoresis, which DNA fragments travel furthest?

Smaller fragments

What do identical DNA profile patterns indicate?

Samples from same person

What does DNA sequencing determine?

Exact order of nucleotides

How long does sequencing a human genome take today?

Just hours

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