Process and Methods (Leaving Cert Biology): Quizzes
📚Quizzes
Practise the questions
12 questions from this quiz
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Practise the questions
12 questions from this quiz
What is another name for genetic engineering?
What is another name for genetic engineering?
Genetic modification
What is recombinant DNA?
What is recombinant DNA?
DNA combining material from different sources
What are the five steps of traditional genetic engineering in correct order?
What are the five steps of traditional genetic engineering in correct order?
DNA iso → Cut → Ligate → Transform → Expr.
What shape are plasmids?
What shape are plasmids?
Circular and small
Why must the same restriction enzyme cut both the target gene and plasmid?
Why must the same restriction enzyme cut both the target gene and plasmid?
To create compatible sticky ends
What are sticky ends?
What are sticky ends?
Single-stranded DNA overhangs
What enzyme acts as molecular glue during ligation?
What enzyme acts as molecular glue during ligation?
DNA ligase
What percentage of bacterial cells typically take up recombinant plasmids during transformation?
What percentage of bacterial cells typically take up recombinant plasmids during transformation?
1-10%
What are genetically modified bacteria called during the expression stage?
What are genetically modified bacteria called during the expression stage?
Biological factories
What does guide RNA do in CRISPR-Cas9?
What does guide RNA do in CRISPR-Cas9?
Directs Cas9 to target location
What key advantage does CRISPR have over traditional methods?
What key advantage does CRISPR have over traditional methods?
Edits genes directly in target cells
Which human protein can bacteria produce through genetic modification?
Which human protein can bacteria produce through genetic modification?
Insulin