Recombinant DNA Technology (AQA A-Level Biology): Flashcards

Producing copies of a specific gene within living organisms

Single-stranded DNA overhangs from staggered restriction cuts

Joins DNA fragments permanently by bonding the backbone

DNA combining genetic material from different sources

Binding site for RNA polymerase to initiate transcription

Signals RNA polymerase to stop transcription

Small, circular DNA in bacteria used as vectors

Process of introducing plasmids into bacterial cells

Produce identifiable traits to find successful transformants

Successful transformants don't fluoresce (gene disrupted)