Producing DNA Fragments (AQA A-Level Biology): Flashcards
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10 cards from this deck
Recombinant DNA
Recombinant DNA
Genetic material combining DNA from two different organisms
Transgenic organism
Transgenic organism
Organism containing recombinant DNA (also called GMO)
Why genes transfer between organisms successfully
Why genes transfer between organisms successfully
Universal genetic code - same in all living organisms
Reverse transcriptase function
Reverse transcriptase function
Enzyme producing DNA from RNA template
cDNA (complementary DNA)
cDNA (complementary DNA)
DNA synthesised from mRNA by reverse transcriptase
Restriction endonuclease function
Restriction endonuclease function
Enzyme cutting DNA at specific recognition sequences
Recognition sequence properties
Recognition sequence properties
Palindromic, 4-8 base pairs long
Sticky ends advantage
Sticky ends advantage
Easily bind to DNA fragments cut with same enzyme
Gene machine
Gene machine
Automated process for artificial gene synthesis in lab
Oligonucleotides
Oligonucleotides
Small overlapping single DNA strands used in gene machine