Skills in A Level Biology (AQA A-Level Biology): Quizzes

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Using an Optical Microscope
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Why are optical microscopes essential tools for biologists?

They magnify structures too small to see

How do optical microscopes create a magnified image?

Light passes through lenses

What are the three standard objective lens magnifications?

×4, ×10, ×40

What is the function of the turret on an optical microscope?

Rotates objective lenses into position

When should you use the coarse focus mechanism?

Initial focusing at low & medium power

Why must specimens be thin or transparent?

To allow light to pass through

What is the purpose of using isotonic solutions?

Prevent osmotic damage to cells

Which stain is used to observe chromosomes during cell division?

Acetic-orcein stain

What is a graticule?

Transparent disc with scale in eyepiece

Why must a graticule be calibrated with a stage micrometer?

Graticule has no fixed units

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