Aseptic techniques (AQA GCSE Biology): Quizzes

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Aseptic techniques
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What is the main purpose of aseptic techniques?

To prevent contamination

How are Petri dishes sterilised at the factory?

Using gamma radiation

Which equipment is used to sterilise culture media like nutrient broth and agar gel?

Autoclave

How are wire loops sterilised in the laboratory?

By heating in Bunsen burner flame

What process do bacteria use to reproduce?

Binary fission

Under ideal conditions, how often can bacteria divide?

Every 20 minutes

Why should you never blow on a hot wire loop to cool it?

It introduces bacteria from breath

What temperature do school laboratories use for incubating bacteria?

25°C

Why do schools incubate bacterial cultures at 25°C rather than 37°C?

It reduces risk of harmful bacteria

If bacteria divide every 30 min, starting with 100, how many after 3 hours?

6,400 bacteria

What do antiseptics do?

Kill or stop growth of microorganisms

How should you open a Petri dish during inoculation?

Open upside down near a flame

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