Purity, Formulations & Chromatography (AQA GCSE Chemistry Combined Science): Flashcards

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Required practical - Chromatography
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Purpose of paper chromatography

Separates and identifies substances in a mixture

What Rf values are used for

To identify substances

Stationary phase in chromatography

Chromatography paper

Mobile phase in chromatography

The solvent

Required safety gear for chromatography

Eye protection

What to use to draw the start line

Pencil and ruler

Solvent level position vs start line

Below the start line

Solvent front definition

The highest point the solvent reaches on the paper

Formula for RfR_f value

Rf=distance moved by substancedistance moved by solventR_f = \frac{\text{distance moved by substance}}{\text{distance moved by solvent}}

Range of RfR_f values

Between 0 and 1

Number of spots from a pure substance

Only one spot

Number of spots from a mixture

Multiple spots

Why not use ink pen for start line

Ink will run

Substances traveling further are attracted to

The solvent

When to mark the solvent front

Immediately - it disappears when paper dries

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