Locating Genes (AQA A-Level Biology): Flashcards
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Practise the cards
10 cards from this deck
What is RNAi used for?
What is RNAi used for?
Locating and studying gene function
What is siRNA?
What is siRNA?
Small interfering RNA molecules that target specific genes
Typical size of siRNA
Typical size of siRNA
20-25 nucleotides
RISC full form
RISC full form
RNA-induced silencing complex
siRNA function in gene targeting
siRNA function in gene targeting
Guides complex to complementary mRNA sequences
Effect of siRNA on target mRNA
Effect of siRNA on target mRNA
mRNA is degraded, silencing the gene
PCR role in gene location
PCR role in gene location
Amplifies DNA to create templates for gene detection
In vitro transcription product from DNA template
In vitro transcription product from DNA template
Double-stranded RNA
PCR amplification capability
PCR amplification capability
Increases DNA concentrations by millions of times
Specificity requirement in RNA-based methods
Specificity requirement in RNA-based methods
Must target only intended genes to avoid false results