Locating Genes (AQA A-Level Biology): Quizzes

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Locating Genes
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What cuts large double-stranded RNA into siRNA?

Enzymes

What is the typical size range of siRNA fragments?

20-25 nucleotides

What does RISC stand for in gene silencing?

RNA-induced silencing complex

What happens to target mRNA during RNA interference?

It is degraded

In which organisms does the RNA-based approach for gene location work?

Eukaryotes and some prokaryotes

By how much can PCR increase DNA concentrations?

Millions of times

How is double-stranded RNA produced for gene location?

In vitro transcription using PCR

How do scientists determine gene function using RNAi?

By observing effects when genes are silenced

What advantage does combining PCR with RNA-based detection provide?

High sensitivity and specificity

Which factor requires RNA-based methods to target only intended genes?

Specificity

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