Physical Chemistry Practicals (A Level only) (AQA A-Level Chemistry): Flashcards

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Required Practical 7
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Aim of Required Practical 7

Investigate pigments from plant leaves using paper chromatography

Pencil line position on filter paper

1 cm above the bottom

Acetone drops for pigment extraction

Approximately 20 drops

Solvent level position in beaker

Below the pencil line

RfRf value formula

Pigment distance ÷ solvent front distance

Higher RfRf value indicates

More soluble in solvent or less affinity for paper

How affinity affects pigment mobility

Lower affinity to paper means travels further

How solubility affects pigment mobility

More soluble in solvent means travels further

Method to identify pigments

Compare RfRf values to known database values

When to mark solvent front

Immediately after removing paper

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