Required practical - Investigating enzymes (AQA GCSE Biology Combined Science): Quizzes

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Required practical - Investigating enzymes
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What does this practical investigate?

Effect of pH on amylase activity

What does the enzyme amylase do?

Breaks down starch into simple sugars

How much of each solution should be added to the test tube?

2 cm³ of each solution

How often should you test a drop on the spotting tile?

Every 30 seconds

What indicates that all the starch has been digested?

Iodine no longer changes colour

What is the purpose of buffer solutions in this experiment?

To keep pH steady during experiment

What is the formula for calculating rate of reaction?

Rate = 1time\frac{1}{\text{time}}

If a reaction takes 200 seconds, what is the rate of reaction in s⁻¹?

0.005 s⁻¹

What is meant by the optimum pH?

Where the rate is highest

Why must temperature be controlled in this experiment?

So rate changes are only due to pH

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