Investigating microbial cultures (AQA GCSE Biology): Flashcards

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Investigating microbial cultures
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Antiseptics definition

Chemicals that kill/stop growth of microorganisms

Zones of inhibition

Clear areas around discs where bacteria can't grow

Incubation temp. and duration

25°C for 2 days

Why incubate at 25°C

Safe temp; allows growth but not harmful bacteria

How to sterilise forceps

Pass ends through Bunsen burner flame

Measuring zones of inhibition

Two diameter measurements at 90° to each other

Area formula for clear zone

area=πr2\text{area} = \pi r^2

Finding radius from diameter

Divide diameter by 2

Meaning of larger clear zone

More effective antiseptic

Purpose of adhesive tape

Stops harmful bacteria getting out

First safety step before practical

Wipe bench with disinfectant

How many antiseptics tested

Three

Mean diameter calculated from

Two measurements at 90°

Bacterial culture commonly used

E. coli

Why larger zone shows effectiveness

Stops bacteria growing over bigger area

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