Digestion (AQA GCSE Biology): Flashcards

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Food testing
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What do qualitative tests show?

IF a substance is present, not how much

Reagent used to test for reducing sugars

Benedict's solution

Reagent used to test for starch

Iodine solution

Reagent used to test for lipids

Sudan III stain

Reagent used to test for proteins

Biuret reagent

Benedict's test positive color change

Blue to green/orange/red

Iodine test positive color change

Orange-brown to blue-black

Sudan III test positive result

Red upper layer forms

Biuret test positive color change

Blue to pink or purple

Examples of reducing sugars

Glucose and fructose

How to prepare food samples

Mix with 2cm³ distilled water; cut/grind large pieces first

Benedict's test heating time

5 minutes in hot water bath

Key safety property of ethanol

Highly flammable

Safer heating method than Bunsen burner

Hot water bath

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