Required Practical: Enzymes (OCR GCSE Biology A, Combined (Gateway Science Suite)): Flashcards

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Required Practical: Enzymes
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Role of enzymes in body

Biological catalysts that speed up reactions

What amylase breaks down

Starch into maltose

Iodine solution tests for

Starch

Testing interval in practical

Every 30 seconds

Iodine color when starch fully broken down

No longer turns blue-black

Optimum temperature for human enzymes

Around 37°C

What happens beyond optimum temp

Enzyme becomes denatured

Reaction rate after denaturation

Decreases sharply

Solutions used to control pH

pH buffer solutions

When enzyme reaction fastest

At optimum pH

Effect of pH deviations

Can cause enzyme denaturation

Optimum conditions for enzymes

Specific temperature and pH

Temps tested in experiment

0°C, 20°C, 40°C, 60°C, 80°C

pH values tested in experiment

pH 4, 6, 7, 8, 10

Reaction rate as temp increases to optimum

Increases

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