Culturing Microorganisms (OCR GCSE Biology A (Gateway Science Suite)): Quizzes

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Culturing Microorganisms
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What does the culture medium contain to provide energy for microorganisms?

Carbohydrates

In nutrient broth method, why is the flask stoppered with cotton wool?

To prevent air contamination

What do bacteria form when grown on an agar gel plate?

Colonies

How are inoculating loops sterilised?

Passing through a flame

Why should the Petri dish be stored upside down?

Prevents condensation disrupting growth

Why should cultures be incubated at 25 degrees rather than 37 degrees?

37°C allows harmful human bacteria growth

Why should the Petri dish lid not be completely sealed with tape?

Prevents harmful anaerobic bacteria

How fast can bacteria multiply by binary fission?

As fast as every 20 minutes

What is the formula for calculating bacterial population growth?

Bacteria at start × 2^divisions

In antibiotic testing, what should the control disc be soaked in?

Sterile water

What does a bigger zone of inhibition indicate?

More effective antibiotic

What formula is used to calculate cross-sectional areas of colonies or inhibition zones?

πr2πr^2

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