Culturing Microorganisms (OCR GCSE Biology A (Gateway Science Suite)): Flashcards
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15 cards from this deck
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Practise the cards
15 cards from this deck
Culture medium contents
Culture medium contents
Carbohydrates, minerals, proteins, vitamins
Two methods to grow microorganisms in lab
Two methods to grow microorganisms in lab
Nutrient broth solution & agar gel plate
What bacteria form on agar surface
What bacteria form on agar surface
Colonies
Tool used to spread microorganisms on agar
Tool used to spread microorganisms on agar
Inoculating loops
Why sterilise Petri dishes & culture media
Why sterilise Petri dishes & culture media
Prevent contamination/competition/new pathogens
How to sterilise inoculating loops
How to sterilise inoculating loops
Pass through a flame
Why partially (not fully) seal Petri dish
Why partially (not fully) seal Petri dish
Stop airborne microbes but allow oxygen in
Why store Petri dish upside down
Why store Petri dish upside down
Prevent condensation disrupting growth
Incubation temperature for culture
Incubation temperature for culture
25 degrees Celsius
Why not incubate at 37°C
Why not incubate at 37°C
Body temp - harmful bacteria could grow
How bacteria multiply
How bacteria multiply
Binary fission
How often binary fission can occur
How often binary fission can occur
Every 20 minutes
Bacterial population growth formula
Bacterial population growth formula
start = end
What zone of inhibition shows
What zone of inhibition shows
Antibiotic effectiveness (bigger = more effective)
Formula for cross-sectional area
Formula for cross-sectional area
where is radius