In Vitro Gene Cloning (AQA A-Level Biology): Flashcards
📚Flashcards
Practise the cards
10 cards from this deck
ShowHide
Practise the cards
10 cards from this deck
What is in vitro gene cloning?
What is in vitro gene cloning?
Copying DNA fragments outside cells using PCR
Four essential PCR components
Four essential PCR components
DNA fragment, polymerase, primers, nucleotides
Why Taq polymerase used in PCR
Why Taq polymerase used in PCR
Thermostable (stable at high temps)
Role of primers in PCR
Role of primers in PCR
Starting points for DNA polymerase
PCR Stage 1: temp & process
PCR Stage 1: temp & process
95°C - denaturation (DNA strands separate)
PCR Stage 2: temp & process
PCR Stage 2: temp & process
55°C - primer annealing
PCR Stage 3: temp & process
PCR Stage 3: temp & process
72°C - DNA synthesis
What does a thermocycler do?
What does a thermocycler do?
Varies temperature precisely over set time periods
PCR copies after 25 cycles
PCR copies after 25 cycles
Over a million copies
In vitro cloning speed vs in vivo
In vitro cloning speed vs in vivo
Much faster - hours vs days/weeks