Culturing Microorganisms (AQA GCSE Biology): Flashcards

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Culturing Microorganisms
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Culturing microorganisms

Growing them in lab using nutrients

Contents of culture medium

Carbohydrates, minerals, proteins, vitamins

Inoculating loops

Wire loops used to spread microorganisms on agar

Why store Petri dish upside down

Prevents condensation disrupting growth

Why sterilise Petri dishes & media

Prevent contamination from other microorganisms

How to sterilise inoculating loops

Pass them through a flame

Why not completely seal Petri dish

Would allow harmful anaerobic bacteria to grow

Incubation temperature for cultures

25 degrees

Why 25°C not 37°C for incubation

37°C allows harmful bacteria growth (body temp)

Binary fission

Bacteria multiplication - one splits into two

Fastest bacterial division time

Every 20 minutes

Bacteria population formula

Start × 2divisions2^{divisions} = end

Control disc in antibiotic test

Disc soaked in sterile water

Inhibition zone

Clear area where bacteria died around antibiotic disc

Formula for cross-sectional area

πr2\pi r^2 where rr is radius

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