Culturing Microorganisms (AQA GCSE Biology): Quizzes

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Culturing Microorganisms
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What does the culture medium contain to provide energy for microorganisms?

Carbohydrates

In nutrient broth, why is the flask stoppered with cotton wool?

To prevent air contaminating it

What are inoculating loops used for?

Spreading microorganisms over agar

Why must Petri dishes be sterilised before use?

Prevent contamination from other microbes

How are inoculating loops sterilised?

Passing through a flame

Why shouldn't the Petri dish lid be sealed all the way around?

Harmful anaerobic bacteria would grow

Why is the Petri dish stored upside down?

Prevent condensation disrupting growth

Why are cultures incubated at 25°C rather than 37°C?

Prevent harmful human bacteria growing

What is binary fission?

One bacterium splitting into two

How fast can bacteria multiply by binary fission?

As fast as every 20 minutes

What should the control disc be soaked in when testing antibiotics?

Sterile water

What does a larger zone of inhibition indicate?

More effective antibiotic

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