Organisation: Digestion (AQA GCSE Biology): Flashcards

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Required Practical: Enzymes
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Role of enzymes

Biological catalysts that speed up reactions

Product when amylase breaks down starch

Maltose

Solution used to test for starch

Iodine solution

Iodine colour with starch present

Blue-black

Testing frequency in enzyme practical

Every 30 seconds

Optimum temp for human enzymes

Around 37°C

What happens beyond optimum temp

Enzyme becomes denatured

Effect of temp up to optimum

Rate of reaction increases

Optimum conditions definition

Specific temp & pH where enzymes work best

Effect of pH deviation from optimum

Can denature the enzyme

Iodine not turning blue-black indicates

Starch has been fully broken down

Temps used in practical (°C)

0, 20, 40, 60, 80

Effect of temp beyond optimum on rate

Rate decreases sharply

pH levels used in practical

4, 6, 7, 8, 10

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