Microscopy (OCR GCSE Biology A, Combined (Gateway Science Suite)): Flashcards

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Microscopy
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Who first observed cells in 1665?

Robert Hooke

Maximum magnification of a light microscope?

×2000

Resolving power of a light microscope?

200nm

What can light microscopes view?

Tissues, cells, large sub-cellular structures

When were electron microscopes developed?

1930s

What do electron microscopes use instead of light?

Electrons

Sub-cellular structures viewed with electron microscope

Mitochondria, ribosomes, chloroplasts, plasmids

What type of images does SEM create?

3D images

What type of images does TEM create?

2D images

Maximum magnification of an electron microscope?

×2,000,000

Resolving power of SEM?

10nm

Resolving power of TEM?

0.2nm

How to calculate light microscope magnification?

Eyepiece magnification × objective magnification

How to calculate size of object from image?

Size of image / Magnification

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