Ultraviolet-Visible Spectrophotometry (HSC SSCE Chemistry): Flashcards

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Ultraviolet-Visible Spectrophotometry
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UV region wavelength range (nm)

190-400 nm

Visible region wavelength range (nm)

400-800 nm

Chromophores

Groups of atoms that absorb UV-visible light

Beer-Lambert Law equation

A=εlcA = \varepsilon lc

ε\varepsilon in Beer-Lambert Law

Molar absorptivity (units: L mol1cm1\text{L mol}^{-1} \text{cm}^{-1})

Units of absorbance

No units (logarithmic ratio)

λmax\lambda_{\text{max}}

Wavelength of maximum absorbance

Relationship: absorbance & concentration

Directly proportional (AcA \propto c)

Basic principle of UV-vis spectrophotometry

Electron excitation by light energy

Industries using UV-vis spectrophotometry

Pharmaceutical, dye, paint, ink industries

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