In Vivo Gene Cloning (AQA A-Level Biology): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
ShowHide
Practise the questions
10 questions from this quiz
What is in vivo gene cloning?
What is in vivo gene cloning?
Producing gene copies in living organisms
What are sticky ends?
What are sticky ends?
Single-stranded overhangs from staggered cuts
What is the role of DNA ligase in gene cloning?
What is the role of DNA ligase in gene cloning?
Joins DNA by forming phosphate-sugar bonds
Why must a promoter region be added to a DNA fragment?
Why must a promoter region be added to a DNA fragment?
For RNA polymerase to bind and start making mRNA
What is the function of a terminator region?
What is the function of a terminator region?
Signals RNA polymerase to stop transcription
What are plasmids?
What are plasmids?
Small circular DNA in bacteria
How do calcium ions help in transformation?
How do calcium ions help in transformation?
Make bacterial membrane temporarily permeable
What percentage of bacterial cells typically take up plasmids during transformation?
What percentage of bacterial cells typically take up plasmids during transformation?
About 1%
How do antibiotic resistance markers identify successful transformants?
How do antibiotic resistance markers identify successful transformants?
Cells with plasmids survive on antibiotic medium
In fluorescent marker systems, what happens to successful transformants?
In fluorescent marker systems, what happens to successful transformants?
They do not fluoresce