Investigating enzymes (AQA GCSE Biology): Quizzes

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Investigating enzymes
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What is an enzyme?

A protein that speeds up reactions

What does amylase break down?

Starch into simple sugars

Why is iodine solution used in the practical?

It changes colour when starch is present

What is the function of buffer solutions in this experiment?

Keep the pH steady

What is the first step in the practical?

Add iodine to each well in the spotting tile

How much amylase solution is used in the test tube?

2 cm³

How often should you transfer a drop to the spotting tile?

Every 30 seconds

When should you stop the experiment?

When iodine stops changing colour

What is the formula for rate of reaction?

1time (s)\frac{1}{\text{time (s)}}

If a reaction takes 200 seconds, what is the rate?

0.005 /s

What does the optimum pH mean?

pH that gives greatest rate of reaction

What does a short reaction time indicate?

Enzyme working well

What should be on the vertical axis of the graph?

Rate of reaction

Why must all solutions be kept at the same temperature?

Temperature affects enzyme activity

Why are spotting tiles used in this practical?

Test small samples without affecting reaction

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