Agricultural Applications of CRISPR-Cas9 (VCE SSCE Biology): Flashcards

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Agricultural Applications of CRISPR-Cas9
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CRISPR full form

Clustered Regularly Interspaced Short Palindromic Repeats

Cas9 function

Endonuclease that cuts DNA at sites specified by guide RNA

Agricultural productivity needed by 2050

Must almost double

Photorespiration

Wasteful process initiated by Rubisco that limits photosynthesis

C3 plants

Plants with no evolved adaptation to minimise photorespiration

C4 plants photorespiration mechanism

Separate initial carbon fixation & Calvin cycle over space

Target crops for CRISPR-Cas9 (cereals)

Wheat, rice, barley

Rice yield increase from CRISPR (China)

25-30% increase via hormone modification

Mushroom shelf life extension from CRISPR (USA)

30% extension by deleting browning gene

Wheat gluten reduction from CRISPR (Spain)

85% reduction in immunoreactivity to gluten

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