Microscope Measurements & Calculations (AQA A-Level Biology): Flashcards

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Microscope Measurements & Calculations
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Eyepiece graticule definition

Glass disc in eyepiece with etched scale for measuring specimens

Eyepiece graticule dimensions

10mm total length, 100 divisions

Why must eyepiece graticule be calibrated?

Each objective lens magnifies differently, changing what each division represents

Stage micrometer definition

Specialised slide with etched scale of known measurements for calibration

Stage micrometer smallest division

0.01mm (or 10μ\mum)

Calibration needed for each...

Objective lens

Magnification formula

Image sizeActual size\frac{\text{Image size}}{\text{Actual size}}

Actual size formula

Image sizeMagnification\frac{\text{Image size}}{\text{Magnification}}

1mm in μ\mum

1,000μ\mum

Effect of 10× higher magnification on graticule value

Graticule value divides by 10

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