Aseptic Techniques (AQA A-Level Biology): Flashcards
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Aseptic techniques
Aseptic techniques
Methods to prevent contamination by unwanted microorganisms
Purpose of aseptic techniques
Purpose of aseptic techniques
Ensure data is reliable and repeatable
Inhibition zones
Inhibition zones
Clear areas around antimicrobial where bacteria can't grow
Bunsen burner role in aseptic technique
Bunsen burner role in aseptic technique
Creates convection currents drawing airborne microbes away
Purpose of flame sterilisation
Purpose of flame sterilisation
Kills microorganisms on equipment surface
Incubation temp for bacterial plates
Incubation temp for bacterial plates
25°C to prevent pathogenic bacteria growth
Why invert agar plates during incubation
Why invert agar plates during incubation
Prevents condensation dripping onto bacterial culture
Larger inhibition zones indicate
Larger inhibition zones indicate
More effective antimicrobials
Broth in microbiology
Broth in microbiology
Liquid medium with bacterial culture and nutrients
Why not seal agar plates completely
Why not seal agar plates completely
Sealing blocks oxygen and promotes anaerobic bacteria growth