Dehydrogenase Activity in Chloroplasts (AQA A-Level Biology): Quizzes
📚Quizzes
Practise the questions
10 questions from this quiz
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Practise the questions
10 questions from this quiz
What color change occurs when DCPIP is reduced during this practical?
What color change occurs when DCPIP is reduced during this practical?
Blue to colorless
What role do dehydrogenase enzymes play in the light-dependent reactions?
What role do dehydrogenase enzymes play in the light-dependent reactions?
Facilitate electron acceptance by NADP
What happens to chloroplasts during centrifugation at high speed for 10 minutes?
What happens to chloroplasts during centrifugation at high speed for 10 minutes?
They form a pellet at the bottom
Which filter should be used in the colorimeter for this practical?
Which filter should be used in the colorimeter for this practical?
Red filter
Why must chloroplast preparations be kept on ice throughout the experiment?
Why must chloroplast preparations be kept on ice throughout the experiment?
Prevent enzyme denaturation
How often should absorbance readings be recorded during the practical?
How often should absorbance readings be recorded during the practical?
Every 2 minutes for 10 minutes
If the distance from the light source is doubled, what happens to light intensity?
If the distance from the light source is doubled, what happens to light intensity?
Reduces to one-quarter
What does a steeper gradient on the absorbance-time graph indicate?
What does a steeper gradient on the absorbance-time graph indicate?
Higher dehydrogenase activity
How does lower light intensity affect DCPIP reduction?
How does lower light intensity affect DCPIP reduction?
Reduces photoionisation rate
What safety equipment is essential when handling DCPIP?
What safety equipment is essential when handling DCPIP?
Eye protection