Chromatography of Photosynthetic Pigments (AQA A-Level Biology): Quizzes

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Chromatography of Photosynthetic Pigments
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What is the stationary phase in chromatography of photosynthetic pigments?

The chromatography paper

How is the RfR_f value calculated?

Distance by pigment ÷ distance by solvent

Why must a pencil be used instead of pen to mark the chromatography paper?

Ink interferes with results

How many drops of acetone are added to extract pigments from the leaf sample?

Exactly 20 drops

Which pigment typically has the highest RfR_f value?

Carotenes

What happens to pigments with lower affinity to the paper?

They travel further distances

What safety precaution is essential when working with chromatography solvents?

Avoid naked flames

Why must the solvent front be marked immediately after removing the paper?

The boundary fades as solvent evaporates

What adaptation do shade-tolerant plants show in their pigment composition?

Higher chlorophyll b relative to a

Why is the pigment spot reapplied 2-3 times?

To concentrate the pigments

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