Aseptic Techniques (AQA A-Level Biology): Quizzes

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Aseptic Techniques
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What is the main purpose of using aseptic techniques in microbiology experiments?

To prevent contamination by unwanted microbes

What is an inhibition zone in antimicrobial testing?

Clear area where bacterial growth prevented

Why is a Bunsen burner used in the workspace during aseptic techniques?

Creates convection currents drawing microbes away

Why are agar plates inverted during incubation?

To prevent condensation dripping on culture

Why are plates incubated at 25°C rather than 37°C?

To prevent pathogenic bacteria growth

What is the purpose of flaming a wire loop before transferring bacteria?

To kill microorganisms on the equipment

What does a larger inhibition zone indicate about an antimicrobial substance?

It is more effective

Why should agar plates not be completely sealed during incubation?

Prevents oxygen entry, encourages anaerobes

How should a multidisc antibiotic ring be handled when placing it on the agar plate?

By its centre with sterile forceps

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