Calculating Mitotic Index using Plant Cells (AQA A-Level Biology): Quizzes

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Calculating Mitotic Index using Plant Cells
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What does the mitotic index measure in a tissue sample?

Proportion of cells undergoing mitosis

What does it mean that meristematic cells are totipotent?

They can differentiate into any cell type

Why are root tips ideal for investigating mitotic index?

High concentration of dividing cells

What temperature should the HCl be heated to for root tip treatment?

60°C

What is the purpose of treating root tips with HCl?

Softens tissue by breaking down cell walls

Why is toluidine blue O stain used in this practical?

Binds to DNA & makes chromosomes visible

If 45 cells show visible chromosomes out of 520 total cells, what is the mitotic index?

0.087

How many cells should be counted to ensure statistical reliability?

At least 500 cells

Why should the very tip of the root (root cap) be removed?

It has fewer dividing cells

Which magnification should be used first when observing the prepared slide?

Lowest magnification

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