Calculating Mitotic Index using Plant Cells (AQA A-Level Biology): Flashcards
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Definition of mitotic index
Definition of mitotic index
Proportion of cells actively undergoing mitosis in a tissue sample
Meristems
Meristems
Growth regions where plant cells actively divide
Location of meristems in plants
Location of meristems in plants
Shoot and root tips
Property of meristematic cells
Property of meristematic cells
Totipotent - can differentiate into any cell type
Temperature for HCl treatment of root tip
Temperature for HCl treatment of root tip
60°C
Purpose of HCl treatment on root tip
Purpose of HCl treatment on root tip
Softens tissue by breaking down cell walls and middle lamella
Purpose of toluidine blue O stain
Purpose of toluidine blue O stain
Binds to DNA, makes chromosomes visible during mitosis
Mitotic index formula
Mitotic index formula
Minimum cells to count for statistical reliability
Minimum cells to count for statistical reliability
At least 500 cells
What visible chromosomes indicate in cell count
What visible chromosomes indicate in cell count
Active mitosis (cell division)
