Chromatography of Photosynthetic Pigments (AQA A-Level Biology): Flashcards

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Chromatography of Photosynthetic Pigments
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Mobile phase in chromatography

The solvent carrying dissolved pigments

Stationary phase in chromatography

The chromatography paper

RfR_f value formula

Rf=pigment dist.solvent dist.R_f = \frac{\text{pigment dist.}}{\text{solvent dist.}}

Effect of high solubility on pigment travel

Pigment travels further/faster up paper

Effect of low affinity to paper

Pigment travels further, higher RfR_f value

Pigment with highest RfR_f value (~0.9)

Carotenes (orange)

Pigment with lowest RfR_f value (~0.4)

Chlorophyll b (green)

Why use pencil not pen for baseline?

Ink interferes with results and obscures separation

Safety properties of acetone/chromatography solvents

Flammable and cause eye/skin irritation

Shade-tolerant plant pigment adaptation

Higher chlorophyll b concentration

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