Dehydrogenase Activity in Chloroplasts (AQA A-Level Biology): Flashcards
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Role of dehydrogenase in light-dependent reactions
Role of dehydrogenase in light-dependent reactions
Facilitates electron acceptance by NADP
What is DCPIP?
What is DCPIP?
Redox indicator dye
DCPIP colour change when reduced
DCPIP colour change when reduced
Blue to colourless
DCPIP's role in this practical
DCPIP's role in this practical
Artificial electron acceptor competing with NADP
Why keep chloroplast samples on ice
Why keep chloroplast samples on ice
Prevents enzyme denaturation and maintains chloroplast integrity
Colorimeter filter colour used
Colorimeter filter colour used
Red filter
Independent variable investigated
Independent variable investigated
Light intensity (varied by distance)
What steeper gradient on absorbance-time graph shows
What steeper gradient on absorbance-time graph shows
Higher dehydrogenase activity
Effect of lower light intensity on chlorophyll
Effect of lower light intensity on chlorophyll
Reduces rate of photoionisation
When DCPIP remains blue, what happens to absorbance?
When DCPIP remains blue, what happens to absorbance?
Remains higher