Measuring Concentration of Glucose Using a Calibration Curve (AQA A-Level Biology): Flashcards

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Measuring Concentration of Glucose Using a Calibration Curve
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Ion in Benedict's reagent

Cu2+Cu^{2+} ions

Benedict's test colour change

Blue to brick red

Product of Benedict's test with glucose

Insoluble copper(I) oxide

Instrument measuring absorbance

Colorimeter

Y-axis on calibration curve

Absorbance

X-axis on calibration curve

Glucose concentration

Heating temp & time for Benedict's test

90°C90°C for 4 minutes

Filter colour in colorimeter

Red filter

Normal blood glucose range

00.80-0.8 mmol dm3^{-3}

Absorbance vs glucose conc. relationship

Directly proportional

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