Rate of an Enzyme Controlled Reaction (AQA A-Level Biology): Flashcards

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Rate of an Enzyme Controlled Reaction
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Rate of reaction formula

1mean time\frac{1}{\text{mean time}}

Type of enzyme: trypsin

Protease

Visual change when casein hydrolysed

Milk: opaque to colourless

Effect of temp increase before optimum

Rate ↑; more kinetic energy & enzyme-substrate complexes

What happens beyond optimum temperature

Tertiary structure breaks; active site shape changes

Required enzyme-substrate relationship

Complementary

Independent variable investigated

Temperature

Purpose of equilibration

Samples reach target temperature

Graph axes: y vs x

Rate of reaction vs temperature

How to improve reliability

Multiple repeats & calculate mean

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